Anticorps Polyclonal de lapin anti-Carbonic Anhydrase IX/CA9

• Phare
• Validé par KD/KO

Carbonic Anhydrase IX/CA9 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(49)

Avis (5)

N° de cat : 11071-1-AP

Synonymes

CA9, CAIX, carbonic anhydrase IX, CA 9, CA IX

---

Galerie de données de validation

• 

  WB analysis of A549 using 11071-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11071-1-AP (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11071-1-AP

  Non-treated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 11071-1-AP (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11071-1-AP

  WB result of Carbonic Anhydrase IX/CA9 antibody (11071-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Carbonic Anhydrase IX/CA9 transfected A549 cells.

• 

  IP experiment of HeLa using 11071-1-AP

  IP result of anti-Carbonic Anhydrase IX/CA9 (IP:11071-1-AP, 4ug; Detection:11071-1-AP 1:3000) with HeLa cells lysate 1200 ug.

• 

  IHC staining of human stomach cancer using 11071-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11071-1-AP (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11071-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11071-1-AP (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HeLa traitées au chlorure de cobalt
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 27 publications below
• IF: See 9 publications below

Informations sur le produit

11071-1-AP cible Carbonic Anhydrase IX/CA9 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Carbonic Anhydrase IX/CA9 Protéine recombinante Ag1540
• Nom complet: carbonic anhydrase IX
• Masse moléculaire calculée: 459 aa, 50 kDa
• Poids moléculaire observé: 50 kDa, 60-70 kDa
• Numéro d’acquisition GenBank: BC014950
• Symbole du gène: CA9
• Identification du gène (NCBI): 768
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. The protein is present in the cytoplasmic membrane and the nucleus, with a molecular weight of 50 kDa. The molecular weight of the glycosylated form is approximately 60-70 kDa. (PMID: 31142270, PMID: 31819036)

Publications

Species	Application	Title
human	IHC	Proc Natl Acad Sci U S A; CRLX101 nanoparticles localize in human tumors and not in adjacent, nonneoplastic tissue after intravenous dosing.; Authors - Andrew J Clark
human	WB	Cell Commun Signal; KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma; Authors - Mao Shen
human	WB	Biomed Pharmacother; Para-toluenesulfonamide, a novel potent carbonic anhydrase inhibitor, improves hypoxia-induced metastatic breast cancer cell viability and prevents resistance to αPD-1 therapy in triple-negative breast cancer; Authors - Hsin-Yuan Chen
mouse,human	WB,IHC,IF	Oxid Med Cell Longev; Carbonic Anhydrase IX Controls Vulnerability to Ferroptosis in Gefitinib-Resistant Lung Cancer; Authors - Chen Zhang
mouse	IHC	Oxid Med Cell Longev; Targeting the Ang2/Tie2 Axis with Tanshinone IIA Elicits Vascular Normalization in Ischemic Injury and Colon Cancer.; Authors - Wei Zou
mouse	IHC,IF	Eur J Nucl Med Mol Imaging; In vivo three-dimensional evaluation of tumour hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT.; Authors - Wenhui Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Aline Seiko (Verified Customer) (05-12-2025)

It was observed a 70kDa and 25kDa isoform.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Renal cell carcinoma

FH

Maelle (Verified Customer) (01-03-2025)

Lost efficacy after freeze-defreze even if it is in glycerol

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1.250eme
• Cell Tissue Type: liver metastasis of colorectal cancer

FH

Josh (Verified Customer) (10-28-2021)

15 ug of human clear cell renal cell carcinoma cell line RCC4 was resolved on 15% Tris/Glycine and transferred to PVDF membrane. Membrane was blocked in blocking buffer (2% BSA in TBS/0.1% Tween-20) for 1hr at room temperature, followed by overnight incubation with anti-CA9 (1:1000) in blocking buffer at 4oC. After 1hr incubation with anti-Rabbit secondary antibody, membrane was imaged with ECL. Expected band was detected along with several non-specific bands by western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: clear cell renal cell carcinoma cell line RCC4

Carbonic Anhydrase IX/CA9 Antibody Western Blot validation (1:1000 dilution) in clear cell renal cell carcinoma cell line RCC4 (Cat no:11071-1-AP)

FH

K (Verified Customer) (08-03-2021)

total cell lysate (50 ug of human HCC cell lines) was resolved on 10% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in TBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-CA9 in blocking buffer (1:500) at 40C overnight. After 1h incubation with appropriate secondary antibody (anti-Rabbit 1:5000) membranes were imaged with ECL and expected band was detected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HCC cells

Carbonic Anhydrase IX/CA9 Antibody Western Blot validation (1:500 dilution) in HCC cells (Cat no:11071-1-AP)

FH

Paulo (Verified Customer) (03-04-2019)

Total cell lysate (50 ug of human ccRCC cell lines) was resolved on 10% Bis-Tris gel and transferred to PVDF membrane. Membrane was incubated in blocking buffer (5% milk in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-CAIX in blocking buffer (1:250) at 4C overnight. After 1h incubation with appropriate secondary antibody (DAKO anti-Rabbit 1:10000) membranes were imaged with ECL and expected band was detected

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Human kidney cancer cells

{{ptg:RelatedPrimaryAntibodies}}