• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-Carbonic Anhydrase IX/CA9

Carbonic Anhydrase IX/CA9 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(49)

N° de cat : 11071-1-AP

Synonymes

CA9, CAIX, carbonic anhydrase IX, CA 9, CA IX



Applications testées

Résultats positifs en WBcellules A549, cellules HeLa, cellules HeLa traitées au chlorure de cobalt
Résultats positifs en IPcellules HeLa,
Résultats positifs en IHCtissu de cancer de l'estomac humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

11071-1-AP cible Carbonic Anhydrase IX/CA9 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Carbonic Anhydrase IX/CA9 Protéine recombinante Ag1540
Nom complet carbonic anhydrase IX
Masse moléculaire calculée 459 aa, 50 kDa
Poids moléculaire observé50 kDa, 60-70 kDa
Numéro d’acquisition GenBankBC014950
Symbole du gène CA9
Identification du gène (NCBI) 768
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. The protein is present in the cytoplasmic membrane and the nucleus, with a molecular weight of 50 kDa. The molecular weight of the glycosylated form is approximately 60-70 kDa. (PMID: 31142270, PMID: 31819036)

Protocole

Product Specific Protocols
WB protocol for Carbonic Anhydrase IX/CA9 antibody 11071-1-APDownload protocol
IHC protocol for Carbonic Anhydrase IX/CA9 antibody 11071-1-APDownload protocol
IP protocol for Carbonic Anhydrase IX/CA9 antibody 11071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Proc Natl Acad Sci U S A

CRLX101 nanoparticles localize in human tumors and not in adjacent, nonneoplastic tissue after intravenous dosing.

Authors - Andrew J Clark
humanWB

Cell Commun Signal

KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma

Authors - Mao Shen
humanWB

Biomed Pharmacother

Para-toluenesulfonamide, a novel potent carbonic anhydrase inhibitor, improves hypoxia-induced metastatic breast cancer cell viability and prevents resistance to αPD-1 therapy in triple-negative breast cancer

Authors - Hsin-Yuan Chen
mouse,humanWB,IHC,IF

Oxid Med Cell Longev

Carbonic Anhydrase IX Controls Vulnerability to Ferroptosis in Gefitinib-Resistant Lung Cancer

Authors - Chen Zhang
mouseIHC

Oxid Med Cell Longev

Targeting the Ang2/Tie2 Axis with Tanshinone IIA Elicits Vascular Normalization in Ischemic Injury and Colon Cancer.

Authors - Wei Zou
mouseIHC,IF

Eur J Nucl Med Mol Imaging

In vivo three-dimensional evaluation of tumour hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT.

Authors - Wenhui Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Aline Seiko (Verified Customer) (05-12-2025)

It was observed a 70kDa and 25kDa isoform.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: Renal cell carcinoma
FH

Maelle (Verified Customer) (01-03-2025)

Lost efficacy after freeze-defreze even if it is in glycerol

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1.250eme
  • Cell Tissue Type: liver metastasis of colorectal cancer
FH

Josh (Verified Customer) (10-28-2021)

15 ug of human clear cell renal cell carcinoma cell line RCC4 was resolved on 15% Tris/Glycine and transferred to PVDF membrane. Membrane was blocked in blocking buffer (2% BSA in TBS/0.1% Tween-20) for 1hr at room temperature, followed by overnight incubation with anti-CA9 (1:1000) in blocking buffer at 4oC. After 1hr incubation with anti-Rabbit secondary antibody, membrane was imaged with ECL. Expected band was detected along with several non-specific bands by western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: clear cell renal cell carcinoma cell line RCC4
Carbonic Anhydrase IX/CA9 Antibody Western Blot validation (1:1000 dilution) in clear cell renal cell carcinoma cell line RCC4 (Cat no:11071-1-AP)
FH

K (Verified Customer) (08-03-2021)

total cell lysate (50 ug of human HCC cell lines) was resolved on 10% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in TBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-CA9 in blocking buffer (1:500) at 40C overnight. After 1h incubation with appropriate secondary antibody (anti-Rabbit 1:5000) membranes were imaged with ECL and expected band was detected.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HCC cells
Carbonic Anhydrase IX/CA9 Antibody Western Blot validation (1:500 dilution) in HCC cells (Cat no:11071-1-AP)
FH

Paulo (Verified Customer) (03-04-2019)

Total cell lysate (50 ug of human ccRCC cell lines) was resolved on 10% Bis-Tris gel and transferred to PVDF membrane. Membrane was incubated in blocking buffer (5% milk in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-CAIX in blocking buffer (1:250) at 4C overnight. After 1h incubation with appropriate secondary antibody (DAKO anti-Rabbit 1:10000) membranes were imaged with ECL and expected band was detected

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Human kidney cancer cells
{{ptg:RelatedPrimaryAntibodies}}