Anticorps Polyclonal de lapin anti-CACNA1B

CACNA1B Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 19681-1-AP

Synonymes

BIII, Brain calcium channel III, CACH5, CACNA1B, CACNL1A5, CACNN, Cav2.2

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Galerie de données de validation

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  IHC staining of mouse brain using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19681-1-AP (CACNA1B Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19681-1-AP (CACNA1B Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19681-1-AP (CACNA1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 19681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19681-1-AP (CACNA1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

19681-1-AP cible CACNA1B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: calcium channel, voltage-dependent, N type, alpha 1B subunit
• Masse moléculaire calculée: 262 kDa
• Numéro d’acquisition GenBank: NM_000718
• Symbole du gène: CACNA1B
• Identification du gène (NCBI): 774
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CACNA1B, also named as CACH5, CACNL1A5 and BIII, belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. CACNA1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). CACNA1B may play a role in directed migration of immature neurons.

Publications

Species	Application	Title
rat	WB	Mol Neurobiol; Genomic Action of Sigma-1 Receptor Chaperone Relates to Neuropathic Pain.; Authors - Shao-Ming Wang
human	WB	Front Pharmacol; Sigma-1 Receptor Plays a Negative Modulation on N-type Calcium Channel.; Authors - Kang Zhang
human	WB	J Recept Signal Transduct Res; CACNA1B facilitates breast cancer cell growth and migration by regulating cyclin D1 and EMT: the implication of CACNA1B in breast cancer.; Authors - Yong-Mei Jin
human	WB	Cell Death Dis; Cav2.2-NFAT2-USP43 axis promotes invadopodia formation and breast cancer metastasis through cortactin stabilization; Authors - Ying Xue ; KO Validated

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