Anticorps Monoclonal anti-CaMKII alpha

CaMKII alpha Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

1E3B3

N° de cat : 66843-1-Ig

Synonymes

CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMK2, CAMKA, CaMK-II subunit alpha

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Galerie de données de validation

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  WB analysis using 66843-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66843-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66843-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 66843-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 66843-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 66843-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66843-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66843-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66843-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66843-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66843-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66843-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 66843-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CAMK2 antibody (66843-1-Ig, Clone: 1E3B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, tissu cérébral de lapin, tissu cérébral de rat, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de porc, tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

66843-1-Ig cible CaMKII alpha dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CaMKII alpha Protéine recombinante Ag4736
• Nom complet: calcium/calmodulin-dependent protein kinase II alpha
• Masse moléculaire calculée: 478 aa, 54 kDa
• Poids moléculaire observé: 45-60 kDa
• Numéro d’acquisition GenBank: BC040457
• Symbole du gène: CaMKII alpha
• Identification du gène (NCBI): 815
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It has 10 potential isoforms with a molecular mass range from 21 to 63 kDa. This antibody can recognize all the members of the CAMK2 family.

Publications

Species	Application	Title
rat	WB	Aging (Albany NY); Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway.; Authors - Ting-Ting Chen
mouse	IF	Brain Res Bull; Effects of Interleukin-19 overexpression in the medial prefrontal cortex on anxiety-related behaviors, BDNF expression and p38/JNK/ERK pathways; Authors - Yuting Jiang
mouse	WB	Phytomedicine; Zexieyin formula alleviates Alzheimer's disease via post-synaptic CaMKII modulating AMPA receptor: Involved in promoting neurogenesis to strengthen synaptic plasticity in mice hippocampus; Authors - Yan Sun
human	WB	Noncoding RNA Res; LncRNA NEAT1-206 regulates autophagy of human umbilical cord mesenchymal stem cells through the WNT5A/Ca2+ signaling pathway under senescence stress; Authors - Weili Wang
rat	IF	CNS Neurosci Ther; Electroacupuncture Regulates Sympathetic Nerve Through the NTSGlu-RVLM Circuit to Relieve Spontaneous Pain in SNI Rats; Authors - Wen Chen

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