Anticorps Polyclonal de lapin anti-CaMKII alpha

CaMKII alpha Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(32)

N° de cat : 13730-1-AP

Synonymes

CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CAMK2, CAMKA, CaMK-II subunit alpha

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Galerie de données de validation

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  WB analysis using 13730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13730-1-AP (CAMK2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 13730-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13730-1-AP (CAMK2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 13730-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13730-1-AP (CAMK2 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse brain using 13730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CaMKII alpha antibody (13730-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 13730-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CaMKII alpha antibody (13730-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 29 publications below
• IF: See 4 publications below

Informations sur le produit

13730-1-AP cible CaMKII alpha dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CaMKII alpha Protéine recombinante Ag4745
• Nom complet: calcium/calmodulin-dependent protein kinase II alpha
• Masse moléculaire calculée: 478 aa, 54 kDa
• Poids moléculaire observé: 45-60 kDa
• Numéro d’acquisition GenBank: BC040457
• Symbole du gène: CaMKII alpha
• Identification du gène (NCBI): 815
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of the CAMK2 family.

Publications

Species	Application	Title
mouse	WB	Anesthesiology; Nerve Growth Factor/Tyrosine Kinase A Receptor Pathway Enhances Analgesia in an Experimental Mouse Model of Bone Cancer Pain by Increasing Membrane Levels of δ-Opioid Receptors; Authors - Bu-Fan Kan
human	WB	Cancer Lett; MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma.; Authors - Qi Sun
pig	WB	Food Chem; TMT-based quantitative proteomic analysis of porcine muscle associated with postmortem meat quality.; Authors - Xinhua Hou
mouse	WB	J Neuroinflammation; Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model; Authors - Yitian Lu
mouse	WB	Chemosphere; MCLR induces dysregulation of calcium homeostasis and endoplasmic reticulum stress resulting in apoptosis in Sertoli cells.; Authors - Sujuan Zhao
mouse	WB	CNS Neurosci Ther; miR-26a-5p alleviates CFA-induced chronic inflammatory hyperalgesia through Wnt5a/CaMKII/NFAT signaling in mice; Authors - Yitian Lu

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