Anticorps Polyclonal de lapin anti-CaMKII Beta

• Phare
• Validé par KD/KO

CaMKII Beta Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 11533-1-AP

Synonymes

CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2, CAMK2B, CAMKB, CaMKII beta

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Galerie de données de validation

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  WB analysis using 11533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11533-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 11533-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11533-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11533-1-AP

  IP Result of anti-CaMKII beta (IP:11533-1-AP, 4ug; Detection:11533-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 11533-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11533-1-AP (CaMKII beta antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 11533-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11533-1-AP (CaMKII beta antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 11533-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11533-1-AP (CaMKII beta antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

11533-1-AP cible CaMKII Beta dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CaMKII Beta Protéine recombinante Ag2119
• Nom complet: calcium/calmodulin-dependent protein kinase II beta
• Masse moléculaire calculée: 503 aa, 56 kDa
• Poids moléculaire observé: 50-70 kDa
• Numéro d’acquisition GenBank: BC019070
• Symbole du gène: CAMK2B
• Identification du gène (NCBI): 816
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named as CAM2, CAMK2 and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target which can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with the molecular weight between 50 kDa and 73 kDa. This antibody may have cross reaction with CAMK2A/D/G due to the high homology.

Publications

Species	Application	Title
rat	WB	Aging (Albany NY); Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway.; Authors - Ting-Ting Chen
mouse	WB	Sci Total Environ; Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.; Authors - Huanliang Liu
human	WB,IHC	Front Oncol; Anti-Tumor Role of CAMK2B in Remodeling the Stromal Microenvironment and Inhibiting Proliferation in Papillary Renal Cell Carcinoma.; Authors - Qingan Jia ; KD Validated
human	IP	Front Cell Neurosci; Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced Retinal Neuronal Regulated Necrosis.; Authors - Shuchao Wang
mouse	WB	PLoS One; Adaptive Gene Regulation in the Striatum of RGS9-Deficient Mice.; Authors - Kathy Busse
rat	WB,IF	Neural Plast; Ketamine Induces Lasting Antidepressant Effects by Modulating the NMDAR/CaMKII-Mediated Synaptic Plasticity of the Hippocampal Dentate Gyrus in Depressive Stroke Model.; Authors - Idriss Ali Abdoulaye