Anticorps Polyclonal de lapin anti-CAMK2B

• Phare
• Validé par KD/KO

CAMK2B Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 11533-1-AP

Synonymes

Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2

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Galerie de données de validation

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  WB analysis using 11533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11533-1-AP (CAMK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11533-1-AP

  IP result of anti-CaMKII Beta (IP:11533-1-AP, 4ug; Detection:11533-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 11533-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11533-1-AP (CAMK2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11533-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11533-1-AP (CAMK2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 11533-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11533-1-AP (CaMKII beta antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

11533-1-AP cible CAMK2B dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAMK2B Protéine recombinante Ag2119
• Nom complet: calcium/calmodulin-dependent protein kinase II beta
• Masse moléculaire calculée: 503 aa, 56 kDa
• Poids moléculaire observé: 50-70 kDa
• Numéro d’acquisition GenBank: BC019070
• Symbole du gène: CaMKII beta
• Identification du gène (NCBI): 816
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named CAM2, CAMK2, and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target that can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with a molecular weight between 50 kDa and 73 kDa. This antibody may have cross-reaction with CAMK2A/D/G due to the high homology.

Publications

Species	Application	Title
rat	WB	Aging (Albany NY); Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway.; Authors - Ting-Ting Chen
mouse	WB	Sci Total Environ; Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.; Authors - Huanliang Liu
human	WB,IHC	Front Oncol; Anti-Tumor Role of CAMK2B in Remodeling the Stromal Microenvironment and Inhibiting Proliferation in Papillary Renal Cell Carcinoma.; Authors - Qingan Jia ; KD Validated
human	IP	Front Cell Neurosci; Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced Retinal Neuronal Regulated Necrosis.; Authors - Shuchao Wang
mouse	WB	PLoS One; Adaptive Gene Regulation in the Striatum of RGS9-Deficient Mice.; Authors - Kathy Busse
rat	WB,IF	Neural Plast; Ketamine Induces Lasting Antidepressant Effects by Modulating the NMDAR/CaMKII-Mediated Synaptic Plasticity of the Hippocampal Dentate Gyrus in Depressive Stroke Model.; Authors - Idriss Ali Abdoulaye

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