Anticorps Recombinant de lapin anti-CAMP

CAMP Recombinant Antibody for FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230157C6

N° de cat : 82940-2-PBS

Synonymes

CAP18, Antibacterial peptide LL-23, Antibacterial peptide KS-30, Antibacterial peptide KR-20, Antibacterial peptide FF-33

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Galerie de données de validation

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  FC experiment of MCF-7 using 82940-2-RR (same clone as 82940-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human CAMP (82940-2-RR, Clone:230157C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82940-2-PBS in a different storage buffer formulation.

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  FC experiment of Hela using 82940-2-RR (same clone as 82940-2-PBS)

  (blue)1x10^6 Hela cells were intracellularly stained with 0.25 ug Anti-Human CAMP (82940-2-RR, Clone:230157C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution (red), or 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82940-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82940-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82940-2-RR against Human CAMP were performed. The affinity constant is 1.50 nM.

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Informations sur le produit

82940-2-PBS cible CAMP dans les applications de FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CAMP Protéine recombinante Ag2622
• Nom complet: cathelicidin antimicrobial peptide
• Masse moléculaire calculée: 170 aa, 19 kDa
• Numéro d’acquisition GenBank: BC055089
• Symbole du gène: CAMP
• Identification du gène (NCBI): 820
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CAMP is a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. CAMP encodes the 18-kDa proprotein hCAP18. FALL-39 and LL-37 are the mature cathelicidin peptides. This antibody can recognize all the proprotein and cleaved species.

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