Anticorps Polyclonal de lapin anti-CAP1

• Phare
• Validé par KD/KO

CAP1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16231-1-PBS

Synonymes

Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN

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Galerie de données de validation

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  WB analysis using 16231-1-AP (same clone as 16231-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 16231-1-AP (same clone as 16231-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 16231-1-AP (same clone as 16231-1-PBS)

  IP result of anti-CAP1 (IP:16231-1-AP, 3ug; Detection:16231-1-AP 1:500) with A431 cells lysate 1520ug. This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 16231-1-AP (same clone as 16231-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16231-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16231-1-PBS cible CAP1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAP1 Protéine recombinante Ag8876
• Nom complet: CAP, adenylate cyclase-associated protein 1 (yeast)
• Masse moléculaire calculée: 475 aa, 52 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC013963
• Symbole du gène: CAP1
• Identification du gène (NCBI): 10487
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.

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