Anticorps Monoclonal anti-CAPG

• Phare
• Validé par KD/KO

CAPG Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A5B2

N° de cat : 66277-1-PBS

Synonymes

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

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Galerie de données de validation

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  WB analysis of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

  WB result of CAPG antibody (66277-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPG transfected HeLa cells. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66277-1-Ig (same clone as 66277-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 66277-1-Ig (same clone as 66277-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66277-1-Ig (same clone as 66277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66277-1-Ig (same clone as 66277-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66277-1-Ig(CAPG antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66277-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66277-1-PBS cible CAPG dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CAPG Protéine recombinante Ag24676
• Nom complet: capping protein (actin filament), gelsolin-like
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39-40 kDa
• Numéro d’acquisition GenBank: BC000728
• Symbole du gène: CAPG
• Identification du gène (NCBI): 822
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

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