Anticorps Polyclonal de lapin anti-Calpain 3

Calpain 3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28476-1-PBS

Synonymes

CAPN3, Calcium-activated neutral proteinase 3, calpain 3, (p94), Calpain L3, Calpain p94

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Galerie de données de validation

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  WB analysis using 28476-1-AP (same clone as 28476-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28476-1-AP (Calpain 3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28476-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 28476-1-AP (same clone as 28476-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28476-1-AP (CAPN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28476-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28476-1-PBS cible Calpain 3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calpain 3 Protéine recombinante Ag29454
• Nom complet: calpain 3, (p94)
• Poids moléculaire observé: 90-105 kDa, 55-60 kDa
• Numéro d’acquisition GenBank: BC146672
• Symbole du gène: CAPN3
• Identification du gène (NCBI): 825
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).

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