Anticorps Monoclonal anti-CAPN3

CAPN3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G12A5

N° de cat : 67366-1-PBS

Synonymes

1G12A5, Calcium-activated neutral proteinase 3, Calpain 3, calpain 3, (p94), Calpain L3

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Galerie de données de validation

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  WB analysis using 67366-1-Ig (same clone as 67366-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67366-1-Ig (CAPN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67366-1-Ig (same clone as 67366-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67366-1-Ig (CAPN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67366-1-Ig (same clone as 67366-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67366-1-Ig (CAPN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67366-1-Ig (same clone as 67366-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CAPN3 antibody (67366-1-Ig, Clone: 1G12A5 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67366-1-PBS cible CAPN3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CAPN3 Protéine recombinante Ag13179
• Nom complet: calpain 3, (p94)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 90-105 kDa, 55-60 kDa, 30-35 kDa
• Numéro d’acquisition GenBank: BC004883
• Symbole du gène: CAPN3
• Identification du gène (NCBI): 825
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).

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