Anticorps Polyclonal de lapin anti-CAPNS1

• Phare
• Validé par KD/KO

CAPNS1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 25057-1-AP

Synonymes

CAPN4, CAPNS, CANP small subunit, Calpain small subunit 1, Calpain regulatory subunit

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 25057-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25057-1-AP (CAPNS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 25057-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25057-1-AP (CAPNS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25057-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25057-1-AP (CAPNS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 25057-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25057-1-AP (CAPNS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 25057-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25057-1-AP (CAPNS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 25057-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 25057-1-AP (CAPNS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, tissu splénique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

25057-1-AP cible CAPNS1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAPNS1 Protéine recombinante Ag21885
• Nom complet: calpain, small subunit 1
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC011903
• Symbole du gène: CAPNS1
• Identification du gène (NCBI): 826
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
human	WB	J Biol Chem; Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.; Authors - Hui-Yan Lei ; KD Validated
		Oncol Rep; Capn4 promotes epithelial-mesenchymal transition in human melanoma cells through activation of the Wnt/β-catenin pathway.; Authors - Enwen Wang
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu
human	WB,IHC	Int J Clin Exp Pathol; High expression of Capn4 is associated with metastasis and poor prognosis in esophageal squamous cell carcinoma; Authors - Xianxian Wu ; KD Validated
human	WB	Biomed Pharmacother; Cereblon mediates macrophage differentiation and microglial phagocytosis by regulating calpain protease activity; Authors - Liang Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Wei (Verified Customer) (03-12-2020)

CAPNS1 is high expression in mouse heart. It has sharp, correct size band in heart lysates.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

{{ptg:RelatedPrimaryAntibodies}}