Anticorps Polyclonal de lapin anti-CAPZA2

• Phare
• Validé par KD/KO

CAPZA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 15948-1-AP

Synonymes

CAPPA2, CAPZ, CapZ alpha 2, CAPZA2

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Galerie de données de validation

• 

  WB analysis of mouse brain using 15948-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15948-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15948-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15948-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15948-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15948-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 15948-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15948-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15948-1-AP (CAPZA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 15948-1-AP

  IP result of anti-CAPZA2 (IP:15948-1-AP, 4ug; Detection:15948-1-AP 1:800) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 15948-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 15948-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15948-1-AP (CAPZA2 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HepG2, cellules MCF-7, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu hépatique de souris, tissu ovarien de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

15948-1-AP cible CAPZA2 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAPZA2 Protéine recombinante Ag8544
• Nom complet: capping protein (actin filament) muscle Z-line, alpha 2
• Masse moléculaire calculée: 286 aa, 33 kDa
• Poids moléculaire observé: 33-40 kDa
• Numéro d’acquisition GenBank: BC005338
• Symbole du gène: CAPZA2
• Identification du gène (NCBI): 830
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Elife; HIPK4 is essential for murine spermiogenesis.; Authors - J Aaron Crapster
mouse	WB	Oncotarget; Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer.; Authors - Mi Jeong Kwon ; KD Validated
human	WB	Biochem J; Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation.; Authors - João D Santos
mouse	WB	Eur J Cell Biol; Nuclear receptor Nur77 regulates immunomechanics of macrophages; Authors - Sanne C Lith

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