Anticorps Monoclonal anti-CAPZB

CAPZB Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F9G2

N° de cat : 68190-1-PBS

Synonymes

F-actin-capping protein subunit beta, CapZbeta, CapZ beta, 1F9G2

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Galerie de données de validation

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  WB analysis using 68190-1-Ig (same clone as 68190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68190-1-Ig (CAPZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68190-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68190-1-Ig (same clone as 68190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68190-1-Ig (CAPZB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68190-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68190-1-Ig (same clone as 68190-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CAPZB antibody (68190-1-Ig, Clone: 1F9G2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68190-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 68190-1-Ig (same clone as 68190-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CAPZB (68190-1-Ig, Clone:1F9G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68190-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68190-1-PBS cible CAPZB dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CAPZB Protéine recombinante Ag19373
• Nom complet: capping protein (actin filament) muscle Z-line, beta
• Masse moléculaire calculée: 277 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC109241
• Symbole du gène: CAPZB
• Identification du gène (NCBI): 832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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