Anticorps Monoclonal anti-CARD11

CARD11 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3F5H6

N° de cat : 68269-1-PBS

Synonymes

BIMP3, CARD11, Carma 1, CARMA1

---

Galerie de données de validation

• 

  WB analysis using 68269-1-Ig (same clone as 68269-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68269-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 68269-1-Ig (same clone as 68269-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68269-1-PBS in a different storage buffer formulation.

• 

  IF Staining of L02 using 68269-1-Ig (same clone as 68269-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using CARD11 antibody (68269-1-Ig, Clone: 3F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68269-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68269-1-PBS cible CARD11 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CARD11 Protéine recombinante Ag16468
• Nom complet: caspase recruitment domain family, member 11
• Masse moléculaire calculée: 1154 aa, 133 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC111719
• Symbole du gène: CARD11
• Identification du gène (NCBI): 84433
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

{{ptg:RelatedPrimaryAntibodies}}