Anticorps Recombinant de lapin anti-CARHSP1

CARHSP1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230489A2

N° de cat : 83300-3-RR

Synonymes

CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1

---

Galerie de données de validation

• 

  WB analysis using 83300-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83300-3-RR (CARHSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 83300-3-RR

  WB result of CARHSP1 antibody (83300-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARHSP1 transfected HEK-293 cells.

• 

  FC experiment of HepG2 using 83300-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-3-RR, Clone:230489A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HEK-293T, cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83300-3-RR cible CARHSP1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CARHSP1 Protéine recombinante Ag2265
• Nom complet: calcium regulated heat stable protein 1, 24kDa
• Masse moléculaire calculée: 147 aa, 16 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003366
• Symbole du gène: CARHSP1
• Identification du gène (NCBI): 23589
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CARHSP1(calcium-regulated heat-stable protein 1, a 24 kDa protein also known as CRHSP-24) is a ubiquitously expressed phosphoprotein that is comprised of 147 amino acids with nearly 14% proline (PMID: 21990353,30257675). CARHSP1 was discovered as a biomarker for diabetic retinopathy and as a regulator of TNF-α mRNA stability in macrophages (PMID: 21078874). CARHSP1 may be involved in oxidative stress via traffic between stress granules and processing bodies (PMID: 21177848).

{{ptg:RelatedPrimaryAntibodies}}