Anticorps Monoclonal anti-CARS

CARS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F7E4

N° de cat : 67933-1-PBS

Synonymes

CARS, CARS1, CYSRS, Cysteine tRNA ligase, cysteinyl tRNA synthetase

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Galerie de données de validation

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  WB analysis using 67933-1-Ig (same clone as 67933-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

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  WB analysis using 67933-1-Ig (same clone as 67933-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67933-1-Ig (same clone as 67933-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67933-1-Ig (same clone as 67933-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CARS antibody (67933-1-Ig, Clone: 2F7E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67933-1-PBS cible CARS dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CARS Protéine recombinante Ag7651
• Nom complet: cysteinyl-tRNA synthetase
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 70-85 kDa
• Numéro d’acquisition GenBank: BC002880
• Symbole du gène: CARS
• Identification du gène (NCBI): 833
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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