Anticorps Polyclonal de lapin anti-CASK

• Phare
• Validé par KD/KO

CASK Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55277-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 55277-1-AP (same clone as 55277-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 55277-1-AP (same clone as 55277-1-PBS)

  WB result of CASK antibody (55277-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CASK transfected A549 cells. This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 55277-1-AP (same clone as 55277-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 55277-1-AP (same clone as 55277-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 55277-1-AP (same clone as 55277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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  IHC staining of rat small intestine using 55277-1-AP (same clone as 55277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 55277-1-AP (same clone as 55277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55277-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55277-1-PBS cible CASK dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: calcium/calmodulin-dependent serine protein kinase (MAGUK family)
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 100-110 kDa, 50-55 kDa
• Numéro d’acquisition GenBank: NM_003688
• Symbole du gène: CASK
• Identification du gène (NCBI): 8573
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CASK, also named as LIN2, belongs to the MAGUK family. It is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. It contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. CASK binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. It may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) . Defects in CASK are the cause of FG syndrome type 4 (FGS4). This antibody can recognize all the isoform of CASK.

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