Anticorps Polyclonal de lapin anti-Caspase 3/p17/p19

• Phare
• Validé par KD/KO

Caspase 3/p17/p19 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (6)

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1964

Avis (7)

N° de cat : 19677-1-AP

Synonymes

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase 3/p17/p19, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Galerie de données de validation

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  WB analysis using 19677-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19677-1-AP (Caspase 3/p17/p19 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19677-1-AP

  WB result of Caspase 3 antibody (19677-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected Jurkat cells.

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  WB analysis of mouse spleen using 19677-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19677-1-AP (CASP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19677-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19677-1-AP (CASP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19677-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19677-1-AP (Caspase 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 19677-1-AP

  IP result of anti-Caspase 3 (IP:19677-1-AP, 4ug; Detection:19677-1-AP 1:300) with NIH/3T3 cells lysate 3440 ug.

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  IHC staining of mouse brain using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human teeth using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human teeth tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human teeth using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human teeth tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19677-1-AP (CASP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19677-1-AP (CASP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 19677-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 19677-1-AP (Caspase 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 19677-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19677-1-AP (Caspase 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Retinal organoids using 19677-1-AP

  Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Vimentin with 60330-1-Ig at 1:500 dilution (green) and Caspase 3 using 19677-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 20 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

• 

  IF Staining of mouse brain using 19677-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 19677-1-AP (Caspase 3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 19677-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Caspase 3/p17/p19 (19677-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules Jurkat traitées à la staurosporine, tissu cérébral de rat, tissu hépatique de rat, tissu splénique de souris
• Résultats positifs en IP: cellules NIH/3T3,
• Résultats positifs en IHC: tissu cérébral de souris, tissu dentaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3, cellules HeLa, tissu cérébral de souris
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 1808 publications below
• IHC: See 178 publications below
• IF: See 90 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

19677-1-AP cible Caspase 3/p17/p19 dans les applications de WB, RIP, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, Lapin, poisson-zèbre, poulet, singe, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caspase 3, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32-35 kDa, 17 kDa, 19 kDa
• Numéro d’acquisition GenBank: NM_004346
• Symbole du gène: CASP3
• Identification du gène (NCBI): 836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa(PMID:9747872). This antibody can recognize p17, p19 and p32 of Caspase 3.

Publications

Species	Application	Title
human	WB,IHC	Mol Cancer; hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma; Authors - Lei Xu
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
	WB	Neuron; UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.; Authors - Kejing Zhang
human	WB	Bone Res; Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma; Authors - Ming Luo
human,mouse	WB	Theranostics; Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI; Authors - Bojun Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

Reliable apoptosis Ab for IF

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: ROs

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NSC34

FH

Hala (Verified Customer) (04-12-2021)

works very well

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepg2 treated with ethoposide

FH

Isha (Verified Customer) (02-03-2021)

Gave me crystal clear bands in kidney lysates. really happy with the product

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Kidney

FH

Diane (Verified Customer) (02-02-2021)

Incubated overnight 4 degrees C. Secondary 1:2500. Excellent bands. Used Opti-4CN substrate kit for visualization.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Cell lysates - glioblastoma primary

Caspase 3/p17/p19 Antibody Western Blot validation (1:500 dilution) in Cell lysates - glioblastoma primary (Cat no:19677-1-AP)

FH

Chao (Verified Customer) (03-12-2020)

Full-length but not cleaved isoform is detected by western blot

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

FH

Kishor (Verified Customer) (01-30-2019)

It is an excellent antibody, worked every time when I used and got satisfactory results.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

Caspase 3/p17/p19 Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:19677-1-AP)

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