Anticorps Polyclonal de lapin anti-Caspase 6/p18/p11

• Phare
• Validé par KD/KO

Caspase 6/p18/p11 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10198-1-AP

Synonymes

Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase 6/p18/p11, Caspase6, MCH2

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Galerie de données de validation

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  WB analysis of Jurkat using 10198-1-AP

  Staurosporine treat Jurkat cells were subjected to SDS PAGE followed by western blot with 10198-1-AP (Caspase 6/p18/p11 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10198-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10198-1-AP (CASP6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10198-1-AP

  WB result of Caspase 6/p18/p11 antibody (10198-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 6/p18/p11 transfected Jurkat cells.

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  WB analysis of mouse brain using 10198-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10198-1-AP (CASP6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10198-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10198-1-AP (CASP6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 10198-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10198-1-AP (CASP6 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human skin using 10198-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10198-1-AP (CASP6 antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human lung cancer using 10198-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10198-1-AP (CASP6 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 10198-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10198-1-AP (CASP6 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat traitées à la staurosporine, cellules Jurkat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cutané humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below

Informations sur le produit

10198-1-AP cible Caspase 6/p18/p11 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Caspase 6/p18/p11 Protéine recombinante Ag0257
• Nom complet: caspase 6, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 33 kDa, 22 kDa
• Poids moléculaire observé: 33-35 kDa
• Numéro d’acquisition GenBank: BC000305
• Symbole du gène: CASP6
• Identification du gène (NCBI): 839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).

Publications

Species	Application	Title
mouse	WB	Transl Stroke Res; Melatonin Offers Dual-Phase Protection to Brain Vessel Endothelial Cells in Prolonged Cerebral Ischemia-Recanalization Through Ameliorating ER Stress and Resolving Refractory Stress Granule; Authors - Danli Lu
human	WB	Chem Biol Interact; O-Alkylated derivatives of quercetin induce apoptosis of MCF-7 cells via a caspase-independent mitochondrial pathway.; Authors - Han Liao
human	WB	Biotechnol Bioeng; Generation of apoptosis-resistant HEK293 cells with CRISPR/Cas mediated quadruple gene knockout for improved protein and virus production.; Authors - Weifeng Zhang ; KO Validated
human	WB	Front Microbiol; Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.; Authors - Bei Wang
human	WB	Front Pharmacol; Astragalin Inhibits the Proliferation and Migration of Human Colon Cancer HCT116 Cells by Regulating the NF-κB Signaling Pathway.; Authors - Min Yang
rat	WB	Drug Des Devel Ther; The Jieduan-Niwan (JDNW) Formula Ameliorates Hepatocyte Apoptosis: A Study of the Inhibition of E2F1-Mediated Apoptosis Signaling Pathways in Acute-on-Chronic Liver Failure (ACLF) Using Rats.; Authors - Weixin Hou