Anticorps Polyclonal de lapin anti-Caspase 8/p43/p18

• Phare
• Validé par KD/KO

Caspase 8/p43/p18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

288

Avis (2)

N° de cat : 13423-1-AP

Synonymes

Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5

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Galerie de données de validation

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  WB analysis of Jurkat using 13423-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13423-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 13423-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of UV treated Hela using 13423-1-AP

  UV treated Hela cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13423-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 13423-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 13423-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (CASP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13423-1-AP

  IP Result of anti-CASP8 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug.

• 

  IP experiment of Jurkat using 13423-1-AP

  IP result of anti-Caspase 8 (IP:13423-1-AP, 4ug; Detection:13423-1-AP 1:500) with Jurkat cells lysate 2880 ug.

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 13423-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (CASP8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 13423-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 8/p43/p18 antibody (13423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat traitées à la staurosporine, cellules HeLa, cellules Jurkat, cellules Raji, cellules Sp2/0, Hela traitées aux UV, RAW264.7
• Résultats positifs en IP: cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu de lymphome humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 272 publications below
• IHC: See 18 publications below
• IF: See 8 publications below
• IP: See 3 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

13423-1-AP cible Caspase 8/p43/p18 dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, Lapin, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Caspase 8/p43/p18 Protéine recombinante Ag4214
• Nom complet: caspase 8, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 538 aa, 62 kDa
• Poids moléculaire observé: 53-57 kDa, 32-45 kDa
• Numéro d’acquisition GenBank: BC028223
• Symbole du gène: CASP8
• Identification du gène (NCBI): 841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.

Publications

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
	IP	Sci Adv; HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.; Authors - Chun Gao
human	WB	Exp Mol Med; PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer; Authors - Gu-Choul Shin
human	WB	Nat Commun; Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms.; Authors - Christoph Grohmann
human	WB	Aging Dis; MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis.; Authors - Yanhui Cui
human	WB	EMBO J; A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.; Authors - Xu Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaoping (Verified Customer) (12-15-2021)

Got really good bands in kidney lysates at 1:500 dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: kidney

FH

WEI (Verified Customer) (01-17-2020)

Good antibody for detecting IR injury-induced apoptosis in mouse heart.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

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