Anticorps Monoclonal anti-Caspase 8/p43/p18

• Phare
• Validé par KD/KO

Caspase 8/p43/p18 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (5)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

69

CloneNo.

2B9H8

N° de cat : 66093-1-Ig

Synonymes

Apoptotic cysteine protease, Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase 8/p43/p18, Caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5

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Galerie de données de validation

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  WB analysis of Jurkat using 66093-1-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66093-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa cells using 66093-1-Ig

  WB result of CASP8 with normal and apotosis Hela cell. P18 can be get in the apotosis cell. 60kd,50kd and 45kd bands are some isoforms of precursor CASP8.

• 

  WB analysis of HepG2 using 66093-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (CASP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66093-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (CASP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 66093-1-Ig

  IP Result of anti-Caspase 8 (IP:66093-1-Ig, 5ug; Detection:66093-1-Ig 1:500) with HepG2 cells lysate 2400ug.

• 

  IHC staining of human liver cancer using 66093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (CASP8 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 66093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (CASP8 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 66093-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66093-1-Ig(Caspase 8 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 60 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

66093-1-Ig cible Caspase 8/p43/p18 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, poulet, singe, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Caspase 8/p43/p18 Protéine recombinante Ag20524
• Nom complet: caspase 8, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 538 aa, 62 kDa
• Poids moléculaire observé: 53-57 kDa, 32-45 kDa, 18 kDa
• Numéro d’acquisition GenBank: BC028223
• Symbole du gène: CASP8
• Identification du gène (NCBI): 841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.

Publications

Species	Application	Title
human	WB	Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li ; KO Validated
human	WB,IHC	Acta Biomater; TRAIL-modified, doxorubicin-embedded periodic mesoporous organosilica nanoparticles for targeted drug delivery and efficient antitumor immunotherapy.; Authors - Xinyao Feng
mouse	WB	J Pineal Res; Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota.; Authors - Wenkai Ren
human	WB,IF	Chem Sci; Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay.; Authors - Meng Liu
chicken	WB	J Hazard Mater; The role of ATF6 in Cr(VI)-induced apoptosis in DF-1 cells.; Authors - Shuo Zhang
human	WB	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge

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