Anticorps Polyclonal de lapin anti-Calsequestrin 2

• Phare
• Validé par KD/KO

Calsequestrin 2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 18422-1-AP

Synonymes

Calsequestrin 2, CASQ2, PDIB2

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Galerie de données de validation

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  WB analysis of human heart using 18422-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 18422-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18422-1-AP (Calsequestrin 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18422-1-AP (Calsequestrin 2 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of C2C12 using 18422-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed C2C12 cell using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de porc, tissu cardiaque de rat, tissu cardiaque humain, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu cardiaque humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellule C2C12

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

18422-1-AP cible Calsequestrin 2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calsequestrin 2 Protéine recombinante Ag13246
• Nom complet: calsequestrin 2 (cardiac muscle)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC022288
• Symbole du gène: CASQ2
• Identification du gène (NCBI): 845
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

Publications

Species	Application	Title
mouse	WB	EMBO Mol Med; SK4 K(+) channels are therapeutic targets for the treatment of cardiac arrhythmias.; Authors - Shiraz Haron-Khun
mouse	WB,IF	JCI Insight; The Purkinje-myocardial junction is the anatomical origin of ventricular arrhythmia in CPVT.; Authors - Daniel J Blackwell ; KO Validated
mouse	WB,IF	Heart Rhythm; Viral delivered gene therapy to treat catecholaminergic polymorphic ventricular tachycardia (CPVT2) in mouse models.; Authors - Efrat Kurtzwald-Josefson ; KO Validated
human	WB	Stem Cell Reports; A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology.; Authors - Matthew J Birket
		Mol Biol Cell; Fer1l6 Is Essential for the Development of Vertebrate Muscle Tissue in Zebrafish.; Authors - Josephine A Bonventre
mouse	WB	JCI Insight; Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy.; Authors - Yingchao Shi