Anticorps Recombinant de lapin anti-Calsequestrin 2

Calsequestrin 2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241211A3

N° de cat : 84056-6-PBS

Synonymes

CASQ2, 241211A3, Calsequestrin-2

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Galerie de données de validation

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  WB analysis using 84056-6-RR (same clone as 84056-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84056-6-RR (CASQ2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84056-6-RR (same clone as 84056-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84056-6-RR (CASQ2 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84056-6-RR (same clone as 84056-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84056-6-RR (CASQ2 antibody) at dilution of 1:0 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 84056-6-RR (same clone as 84056-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CASQ2 antibody (84056-6-RR, Clone: 241211A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 84056-6-RR (same clone as 84056-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CASQ2 antibody (84056-6-RR, Clone: 241211A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 84056-6-RR (same clone as 84056-6-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug CASQ2 Recombinant antibody (84056-6-RR, Clone:241211A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 84056-6-RR (same clone as 84056-6-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug CASQ2 Recombinant antibody (84056-6-RR, Clone:241211A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84056-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84056-6-RR against Human CASQ2 were performed. The affinity constant is 0.251 nM.

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Informations sur le produit

84056-6-PBS cible Calsequestrin 2 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Calsequestrin 2 Protéine recombinante Ag33584
• Nom complet: calsequestrin 2 (cardiac muscle)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46-55 kDa
• Numéro d’acquisition GenBank: BC022288
• Symbole du gène: Calsequestrin 2
• Identification du gène (NCBI): 845
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

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