Anticorps Recombinant de lapin anti-Calsequestrin 2

Calsequestrin 2 Recombinant Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241211A3

N° de cat : CL488-84056-6

Synonymes

CASQ2, 241211A3, Calsequestrin-2

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Galerie de données de validation

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  IF Staining of C2C12 using CL488-84056-6

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CoraLite® Plus 488 CASQ2 antibody (CL488-84056-6, Clone: 241211A3 ) at dilution of 1:200.

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  FC experiment of U2OS using CL488-84056-6

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Casq2 Recombinant Antibody (CL488-84056-6, Clone:241211A3)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of A549 using CL488-84056-6

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Casq2 Recombinant Antibody (CL488-84056-6, Clone:241211A3)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules C2C12,
• Résultats positifs en FC (Intra): cellules U2OS, cellules A549

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-84056-6 cible Calsequestrin 2 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Calsequestrin 2 Protéine recombinante Ag33584
• Nom complet: calsequestrin 2 (cardiac muscle)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46-55 kDa
• Numéro d’acquisition GenBank: BC022288
• Symbole du gène: Calsequestrin 2
• Identification du gène (NCBI): 845
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

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