Anticorps Recombinant de lapin anti-Calpastatin

Calpastatin Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251652G2

N° de cat : 86664-3-PBS

Synonymes

CAST, Calpain inhibitor, Sperm BS-17 component

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Galerie de données de validation

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  WB analysis using 86664-3-RR (same clone as 86664-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86664-3-RR (CAST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86664-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86664-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86664-3-RR against Human Calpastatin were performed. The affinity constant is 6.39 nM.

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Informations sur le produit

86664-3-PBS cible Calpastatin dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Calpastatin Protéine recombinante Ag2897
• Nom complet: calpastatin
• Masse moléculaire calculée: 667 aa, 72 kDa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: BC013579
• Symbole du gène: Calpastatin
• Identification du gène (NCBI): 831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calpastatin (CAST) is an endogenous protein that specifically inhibits the activity of calpains, a family of calcium-dependent proteases involved in numerous cellular processes. The CAST gene encodes calpastatin, which contains multiple calpain-inhibiting domains that bind to the active site of calpain, preventing it from performing its functions. Calpastatin is found in the cytosol and plays a key role in regulating intracellular proteolysis, with malfunctions in the calpain-calpastatin system linked to various diseases, including autoimmune disorders and neurodegenerative conditions.

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