Anticorps Monoclonal anti-CBFB

CBFB Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D7F2

N° de cat : 67885-1-PBS

Synonymes

1D7F2, CBF beta, CBF-beta, Core-binding factor subunit beta, PEA2-beta

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Galerie de données de validation

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  WB analysis using 67885-1-Ig (same clone as 67885-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67885-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67885-1-Ig (same clone as 67885-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67885-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 67885-1-Ig (same clone as 67885-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CBFB (67885-1-Ig, Clone:1D7F2) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67885-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67885-1-PBS cible CBFB dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CBFB Protéine recombinante Ag31183
• Nom complet: core-binding factor, beta subunit
• Masse moléculaire calculée: 182 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC018509
• Symbole du gène: CBFB
• Identification du gène (NCBI): 865
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.

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