Anticorps Monoclonal anti-CBLB

CBLB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2D3A2

N° de cat : 66353-1-PBS

Synonymes

CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 66353-1-Ig (same clone as 66353-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 66353-1-Ig (same clone as 66353-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 66353-1-Ig (same clone as 66353-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed RAW 264.7 cells using 66353-1-Ig(CBLB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66353-1-PBS cible CBLB dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CBLB Protéine recombinante Ag18757
• Nom complet: Cas-Br-M (murine) ecotropic retroviral transforming sequence b
• Masse moléculaire calculée: 982 aa, 109 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC032851
• Symbole du gène: CBLB
• Identification du gène (NCBI): 868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.

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