Anticorps Polyclonal de lapin anti-CBLL1

• Phare
• Validé par KD/KO

CBLL1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21179-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 21179-1-AP (same clone as 21179-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21179-1-AP (CBLL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 21179-1-AP (same clone as 21179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 21179-1-AP (same clone as 21179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 21179-1-AP (same clone as 21179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21179-1-AP (same clone as 21179-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBLL1 antibody (21179-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21179-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

21179-1-PBS cible CBLL1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CBLL1 Protéine recombinante Ag15463
• Nom complet: Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1
• Masse moléculaire calculée: 491 aa, 55 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC027460
• Symbole du gène: CBLL1
• Identification du gène (NCBI): 79872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cbl proto‐oncogene E3 ubiquitin protein ligase‐like 1, RNF188, Hakai (CBLL1) is an evolutionarily conserved E3 ubiquitin ligase containing a RING‐finger domain. CBLL1 contains a typical RING‐finger, short pTyr‐binding domain, and proline‐rich domain. CBLL1 expression is upregulated in human colon and gastric cancer tissues, and CBLL1 has been reported to induce anchorage‐dependent cell growth. CBLL1 immunostaining was observed in both the nuclei and cytoplasm of cancer cells. (PMID: 31124298, PMID: 31646569, PMID: 21191016)

{{ptg:RelatedPrimaryAntibodies}}