Anticorps Polyclonal de lapin anti-NCBP1/CBP80

• Phare
• Validé par KD/KO

NCBP1/CBP80 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 10349-1-AP

Synonymes

NCBP1, 80 kDa nuclear cap-binding protein, CBP80, NCBP, NCBP 80 kDa subunit

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Galerie de données de validation

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  WB analysis using 10349-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10349-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10349-1-AP

  IP result of anti-NCBP1 (IP:10349-1-AP, 4ug; Detection:10349-1-AP 1:500) with HeLa cells lysate 2480ug.

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  IHC staining of human lung cancer using 10349-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10349-1-AP (NCBP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 10349-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10349-1-AP (NCBP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10349-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10349-1-AP (NCBP1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 3 publications below

Informations sur le produit

10349-1-AP cible NCBP1/CBP80 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCBP1/CBP80 Protéine recombinante Ag0496
• Nom complet: nuclear cap binding protein subunit 1, 80kDa
• Masse moléculaire calculée: 92 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC001450
• Symbole du gène: NCBP1
• Identification du gène (NCBI): 4686
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.

Publications

Species	Application	Title
human	WB	Cell; RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock.; Authors - Fustin Jean-Michel JM ; KD Validated
mouse	WB,IP	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
human	WB	Mol Cell; Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay.; Authors - Hanae Sato
human	WB,IP	Genes Dev; SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.; Authors - Yamashita Akio A
human	WB	Nucleic Acids Res; SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.; Authors - Yuzhang Du
human	WB	Sci Signal; AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay.; Authors - Izumi Natsuko N

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