Anticorps Recombinant de lapin anti-NCBP1/CBP80

NCBP1/CBP80 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240012G8

N° de cat : 83212-4-PBS

Synonymes

NCBP1, NCBP 80 kDa subunit, NCBP, CBP80, 80 kDa nuclear cap-binding protein

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Galerie de données de validation

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  WB analysis using 83212-4-RR (same clone as 83212-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83212-4-RR (CBP80 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83212-4-RR (same clone as 83212-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83212-4-RR (NCBP1/CBP80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBP80 antibody (83212-4-RR, Clone: 240012G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CBP80 Recombinant antibody (83212-4-RR, Clone:240012G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83212-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83212-4-RR against Human CBP80 were performed. The affinity constant is 0.131 nM.

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Informations sur le produit

83212-4-PBS cible NCBP1/CBP80 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NCBP1/CBP80 Protéine recombinante Ag0496
• Nom complet: nuclear cap binding protein subunit 1, 80kDa
• Masse moléculaire calculée: 92 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC001450
• Symbole du gène: NCBP1
• Identification du gène (NCBI): 4686
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.

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