Anticorps Recombinant de lapin anti-CBX1

• Phare
• Validé par KD/KO

CBX1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240075H6

N° de cat : 83248-4-PBS

Synonymes

240075H6, CBX, Chromobox protein homolog 1, Heterochromatin protein p25, HP1 beta

---

Galerie de données de validation

• 

  WB analysis using 83248-4-RR (same clone as 83248-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83248-4-RR (CBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 83248-4-RR (same clone as 83248-4-PBS)

  WB result of CBX1 antibody (83248-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX1 transfected MCF-7 cells. This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cbx1 Recombinant Antibody (83248-4-RR, Clone:240075H6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83248-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83248-4-RR against Human CBX1 were performed. The affinity constant is 0.605 nM.

---

Informations sur le produit

83248-4-PBS cible CBX1 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CBX1 Protéine recombinante Ag34901
• Nom complet: chromobox homolog 1 (HP1 beta homolog Drosophila )
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC002609
• Symbole du gène: CBX1
• Identification du gène (NCBI): 10951
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

{{ptg:RelatedPrimaryAntibodies}}