Anticorps Polyclonal de lapin anti-CBX3

• Phare
• Validé par KD/KO

CBX3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

28

Avis (1)

N° de cat : 11650-2-AP

Synonymes

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein

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Galerie de données de validation

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  WB analysis of mouse spleen using 11650-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11650-2-AP (CBX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11650-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11650-2-AP (CBX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11650-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11650-2-AP (CBX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 11650-2-AP

  IP result of anti-CBX3(IP:11650-2-AP, 4ug; Detection:11650-2-AP 1:3000) with mouse spleen tissue lysate 1560 ug.

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  IHC staining of human colon cancer using 11650-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11650-2-AP (CBX3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 11650-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11650-2-AP (CBX3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11650-2-AP

  Immunofluorescent analysis of HepG2 cells, using CBX3 antibody 11650-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules A375
• Résultats positifs en IP: tissu splénique de souris,
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 23 publications below
• IHC: See 8 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

11650-2-AP cible CBX3 dans les applications de WB, IP, IHC, IF, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CBX3 Protéine recombinante Ag2251
• Nom complet: chromobox homolog 3 (HP1 gamma homolog, Drosophila)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC000954
• Symbole du gène: CBX3
• Identification du gène (NCBI): 11335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

Publications

Species	Application	Title
mouse	IF	Nat Biotechnol; Retrospective analysis of enhancer activity and transcriptome history; Authors - Ruben Boers
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang
human	WB,IF,IP	Adv Sci (Weinh); Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer; Authors - Huaiyuan Liang ; KD Validated
mouse	WB,IP	J Exp Med; ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.; Authors - Longfei Ma
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang
human	WB,IHC,IF	J Cell Biol; Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins.; Authors - Jiang Wei-Qin WQ ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

Strong bands for predicted size.

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart