Anticorps Polyclonal de lapin anti-CBX5

• Phare
• Validé par KD/KO

CBX5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 31109-1-AP

Synonymes

Antigen p25, HP1, HP1 alpha, HP1A

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Galerie de données de validation

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  WB analysis of K-562 using 31109-1-AP

  WB result of CBX5 antibody (31109-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX5 transfected K-562 cells.

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  WB analysis using 31109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31109-1-AP (CBX5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31109-1-AP cible CBX5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CBX5 Protéine recombinante Ag34810
• Nom complet: chromobox homolog 5 (HP1 alpha homolog, Drosophila)
• Masse moléculaire calculée: 191 aa, 22 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC006821
• Symbole du gène: CBX5
• Identification du gène (NCBI): 23468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromobox protein homolog 5 (CBX5), also named heterochromatin protein 1 alpha (HP1a), is a highly conserved nonhistone protein involved in heterochromatin formation and gene silencing in different species including humans.HP1a is a Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). It may interact with lamin-Breceptor. HP1a is involved in the formation of functional kinetochore through interaction with MIS12complex proteins. Phosphorylation of HP1 and LBR during interphase mitosis may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.The HP1a was expressed in nucleus and associates specifically with chromatin during metaphase and anaphase.Recent studies have shown that HP1a is present at many euchromatic sites and positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila (19798443). This antibody is a rabbit polyclonal antibody raised against a full-length human HP1A protein ,and can reacts with the 28kd HP1A protein.

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