Anticorps Monoclonal anti-CC2D1A

• Phare
• Validé par KD/KO

CC2D1A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C11G2

N° de cat : 68334-1-PBS

Synonymes

FREUD 1, AKI1, Akt kinase-interacting protein 1, Coiled-coil and C2 domain-containing protein 1A, Five prime repressor element under dual repression-binding protein 1

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Galerie de données de validation

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  WB analysis using 68334-1-Ig (same clone as 68334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68334-1-Ig (CC2D1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68334-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68334-1-Ig (same clone as 68334-1-PBS)

  WB result of CC2D1A antibody (68334-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CC2D1A transfected HEK-293 cells. This data was developed using the same antibody clone with 68334-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68334-1-Ig (same clone as 68334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68334-1-Ig (CC2D1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68334-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68334-1-Ig (same clone as 68334-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CC2D1A antibody (68334-1-Ig, Clone: 1C11G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68334-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68334-1-PBS cible CC2D1A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CC2D1A Protéine recombinante Ag10363
• Nom complet: coiled-coil and C2 domain containing 1A
• Masse moléculaire calculée: 951 aa, 104 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC064981
• Symbole du gène: CC2D1A
• Identification du gène (NCBI): 54862
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CC2D1A (coiled-coil and C2 domain-containing 1A), also known as Freud-1, Aki1 or TAPE (TBK1-associated protein in endolysosomes), is a evolutionary conserved protein located in different subcellular compartments, including the nucleus, centrosome, and endolysosomes. It acts as a scaffold protein that interacts with various proteins and plays diverse biological roles. Mutations in CC2D1A have been linked to nonsyndromic mental retardation (NMSR) and generate a truncated 85-kDa product. Several isoforms of CC2D1A proteins have also been described: 120 /130-kDa doublet of long isoform and 67 kDa short isoform. The short isoform of CC2D1A has been identified as the predominant isoform in rodent cells, while the long isoform is more abundant in human cells. Recently it has been reported that CC2D1A/TAPE is the first innate immune regulator implicated in both TLR and RLR signaling at a very early step.

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