Anticorps Recombinant de lapin anti-CCAR1

CCAR1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240980C12

N° de cat : 83936-4-PBS

Synonymes

240980C12, CARP 1, CARP1, CARP-1, Death inducer with SAP domain

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Galerie de données de validation

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  WB analysis using 83936-4-RR (same clone as 83936-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83936-4-RR (CCAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 83936-4-RR (same clone as 83936-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83936-4-RR (CCAR1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 83936-4-RR (same clone as 83936-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83936-4-RR (CCAR1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83936-4-RR (same clone as 83936-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (83936-4-RR, Clone: 240980C12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83936-4-PBS in a different storage buffer formulation.

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Informations sur le produit

83936-4-PBS cible CCAR1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCAR1 Protéine recombinante Ag18786
• Nom complet: cell division cycle and apoptosis regulator 1
• Masse moléculaire calculée: 1150 aa, 133 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC132725
• Symbole du gène: CCAR1
• Identification du gène (NCBI): 55749
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cell cycle and apoptosis regulator 1 (CCAR1) was originally identified from a functional screening and implicated in retinoid-induced cell cycle arrest and apoptosis. It has been shown that, in breast cancer cells, CCAR1 regulates apoptosis induced by various agents, including chemotherapeutics adriamycin, etoposide, and EGFR inhibitor. It is also involved in mediating anchorage-independent growth of colon carcinoma cells. In addition, CCAR1 plays the role as a co-activator of nuclear receptors to recruit mediator complex. It was found to associate with components of the Mediator and p160 coactivator complexes and is recruited to endogenous NR target genes. CCAR1 is also a binding partner of b-catenin and assists b-catenin in transcriptional activation of Wnt target genes.

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