Anticorps Monoclonal anti-CCAR2

• Phare
• Validé par KD/KO

CCAR2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D10F8

N° de cat : 66497-1-PBS

Synonymes

cell cycle and apoptosis regulator 2, DBC1, DBC-1, KIAA1967, DBC.1

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Galerie de données de validation

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  WB analysis using 66497-1-Ig (same clone as 66497-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66497-1-Ig (same clone as 66497-1-PBS)

  WB result of CCAR2 antibody (66497-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCAR2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66497-1-Ig (same clone as 66497-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66497-1-Ig (CCAR2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66497-1-Ig (same clone as 66497-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCAR2 antibody (66497-1-Ig, Clone: 2D10F8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 66497-1-Ig (same clone as 66497-1-PBS)

  1X10^6 U2OS cells were intracellularly stained with 0.2 ug Anti-Human CCAR2 (66497-1-Ig, Clone:2D10F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66497-1-PBS cible CCAR2 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCAR2 Protéine recombinante Ag18374
• Nom complet: KIAA1967
• Masse moléculaire calculée: 923 aa, 103 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC018269
• Symbole du gène: CCAR2
• Identification du gène (NCBI): 57805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).

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