Anticorps Monoclonal anti-CCAR2

• Phare
• Validé par KD/KO

CCAR2 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

2D10F8

N° de cat : CL594-66497

Synonymes

CCAR2, cell cycle and apoptosis regulator 2, DBC 1, DBC.1, DBC1, KIAA1967, p30 DBC, Protein KIAA1967

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Galerie de données de validation

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  IF Staining of HeLa using CL594-66497

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated CCAR2 antibody (CL594-66497, Clone: 2D10F8 ) at dilution of 1:100.

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  FC experiment of HeLa using CL594-66497

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CCAR2 (CL594-66497, Clone:2D10F8) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of U2OS using CL594-66497

  1X10^6 U2OS cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CCAR2 (CL594-66497, Clone:2D10F8) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules U2OS, cellules HeLa
• Résultats positifs en cytométrie: cellules U2OS, cellules HeLa

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66497 cible CCAR2 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCAR2 Protéine recombinante Ag18374
• Nom complet: KIAA1967
• Masse moléculaire calculée: 923 aa, 103 kDa
• Numéro d’acquisition GenBank: BC018269
• Symbole du gène: CCAR2
• Identification du gène (NCBI): 57805
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).

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