Anticorps Polyclonal de lapin anti-CCDC12

CCDC12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25138-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 25138-1-AP (same clone as 25138-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25138-1-AP (CCDC12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 25138-1-AP (same clone as 25138-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 25138-1-AP (CCDC12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 25138-1-AP (same clone as 25138-1-PBS)

  IP result of anti-CCDC12 (IP:25138-1-AP, 4ug; Detection:25138-1-AP 1:600) with HeLa cells lysate 2560ug. This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 25138-1-AP (same clone as 25138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25138-1-AP (CCDC12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 25138-1-AP (same clone as 25138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25138-1-AP (CCDC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 25138-1-AP (same clone as 25138-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCDC12 antibody (25138-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 25138-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25138-1-PBS cible CCDC12 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCDC12 Protéine recombinante Ag18223
• Nom complet: coiled-coil domain containing 12
• Masse moléculaire calculée: 166 aa, 19 kDa
• Poids moléculaire observé: 18-25 kDa
• Numéro d’acquisition GenBank: BC020830
• Symbole du gène: CCDC12
• Identification du gène (NCBI): 151903
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCDC12 is a 166aa protein with MW 18-25 kDa (with some modification).

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