Anticorps Recombinant de lapin anti-CCDC134

• Phare
• Validé par KD/KO

CCDC134 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241212F11

N° de cat : 84106-3-PBS

Synonymes

241212F11

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Galerie de données de validation

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  WB analysis using 84106-3-RR (same clone as 84106-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84106-3-RR (CCDC134 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84106-3-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 84106-3-RR (same clone as 84106-3-PBS)

  WB result of CCDC134 antibody (84106-3-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC134 transfected HEK-293 cells. This data was developed using the same antibody clone with 84106-3-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 84106-3-RR (same clone as 84106-3-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug CCDC134 Recombinant antibody (84106-3-RR, Clone:241212F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84106-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01057-1

  Cytometric bead array standard curve of MP01057-1, CCDC134 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84106-1-PBS. Detection antibody: 84106-3-PBS. Standard: Ag21990. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84106-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84106-3-RR against Human CCDC134 were performed. The affinity constant is 1.69 nM.

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Informations sur le produit

84106-3-PBS cible CCDC134 dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCDC134 Protéine recombinante Ag21990
• Nom complet: coiled-coil domain containing 134
• Masse moléculaire calculée: 229 aa, 27 kDa
• Poids moléculaire observé: 25-27 kDa
• Numéro d’acquisition GenBank: BC017693
• Symbole du gène: CCDC134
• Identification du gène (NCBI): 79879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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