Anticorps Polyclonal de lapin anti-CCDC171

CCDC171 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 28433-1-AP

Synonymes

bA536D16.1, bA778P13.1, C9orf93, CCDC171, DKFZp686P12113, FLJ39267, FLJ46740

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Galerie de données de validation

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  WB analysis using 28433-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28433-1-AP (CCDC171 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28433-1-AP cible CCDC171 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCDC171 Protéine recombinante Ag28926
• Nom complet: chromosome 9 open reading frame 93
• Masse moléculaire calculée: 153 kDa
• Poids moléculaire observé: 142 kDa
• Numéro d’acquisition GenBank: NM_173550
• Symbole du gène: CCDC171
• Identification du gène (NCBI): 203238
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (08-03-2022)

U2OS cells were lysates with RIPA buffer. 200ng for condition was loaded on the gel. . Proteins were run on a 12-4% gradient polyacrylamide gel at 180V constant. Gel was transferred on nitrocellulose filter at 300mA constant. Blots were then blocked in 5%milk in TBS-0,1%Tween for 30 min and then incubated with CCDC171 primary antibody (1:1000 dilution). Anti-rabbit -HRP conjugated antibody (1:2000) was used to detect CCDC171 signal (140-5kDa). b-tubulin (55kDa) was used as a loading control. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: U2OS

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS (Human Osteosarcoma) cells

CCDC171 Antibody Western Blot validation (1:1000 dilution) in U2OS (Human Osteosarcoma) cells (Cat no:28433-1-AP)

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