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Anticorps Polyclonal de lapin anti-CCDC28A

CCDC28A Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 14807-1-AP

Synonymes

C6orf80, CCDC28A, CCRL1AP

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using CCDC28A Polyclonal antibody (14807-1-AP)

    WB analysis using 14807-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CCDC28A Polyclonal antibody (14807-1-AP)

    WB analysis of HeLa using 14807-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CCDC28A Polyclonal antibody (14807-1-AP)

    WB analysis of mouse thymus using 14807-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCDC28A Polyclonal antibody (14807-1-AP)

    IF Staining of MCF-7 using 14807-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CCDC28A antibody (14807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCDC28A Polyclonal antibody (14807-1-AP)

    IF Staining of MCF-7 using 14807-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CCDC28A antibody 14807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Applications testées

Résultats positifs en WBcellules A375, cellules HeLa, cellules Jurkat, cellules MCF-7, tissu de thymus de souris
Résultats positifs en IFcellules MCF-7,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

14807-1-AP cible CCDC28A dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène CCDC28A Protéine recombinante Ag6529
Nom complet coiled-coil domain containing 28A
Masse moléculaire calculée 30 kDa
Poids moléculaire observé70 kDa
Numéro d’acquisition GenBankBC000758
Symbole du gène CCDC28A
Identification du gène (NCBI) 25901
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Protocole

Product Specific Protocols
WB protocol for CCDC28A antibody 14807-1-APDownload protocol
IF protocol for CCDC28A antibody 14807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols