Anticorps Polyclonal de lapin anti-CEP112

• Phare
• Validé par KD/KO

CEP112 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 24928-1-AP

Synonymes

CCDC46, Coiled-coil domain-containing protein 46, Centrosomal protein of 112 kDa

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Galerie de données de validation

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  WB analysis of HEK-293 using 24928-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24928-1-AP (CEP112 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24928-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24928-1-AP (CEP112 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MDCK using 24928-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CEP112 antibody (24928-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules MDCK,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

24928-1-AP cible CEP112 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP112 Protéine recombinante Ag21586
• Nom complet: coiled-coil domain containing 46
• Masse moléculaire calculée: 955 aa, 113 kDa
• Poids moléculaire observé: 110-113 kDa
• Numéro d’acquisition GenBank: BC131805
• Symbole du gène: CEP112
• Identification du gène (NCBI): 201134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	Dev Cell; Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.; Authors - Quynh P H Nguyen ; KO Validated
mouse	WB,IF	PLoS Biol; Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins.; Authors - Suchismita Panda
human	WB,IF	Clin Genet; Loss-of-function mutations in centrosomal protein 112 is associated with human acephalic spermatozoa phenotype.; Authors - Yanwei Sha

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jay (Verified Customer) (11-08-2019)

Human RPE1 cell line with Methanol fixationImmunostaining result shows that CEP112 localizes to centrosome.(Red;Cep112, Green; Cep164 for Centrosome marker, Blue;DAPI for Nucleus).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human RPE1

CEP112 Antibody Immunofluorescence, validation (1:100 dilution) in Human RPE1 (Cat no:24928-1-AP)

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