Anticorps Monoclonal anti-CCDC50

CCDC50 Monoclonal Antibody for Indirect ELISA, WB

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Indirect ELISA, WB

Conjugaison

Non conjugué

CloneNo.

2F12G7

N° de cat : 68369-1-PBS

Synonymes

YMER, Protein Ymer, DFNA44, Coiled-coil domain-containing protein 50, C3orf6

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Galerie de données de validation

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  WB analysis using 68369-1-Ig (same clone as 68369-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68369-1-Ig (CCDC50 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 68369-1-Ig (same clone as 68369-1-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.4 ug CCDC50 Monoclonal antibody (68369-1-Ig, Clone:2F12G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68369-1-PBS cible CCDC50 dans les applications de Indirect ELISA, WB et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCDC50 Protéine recombinante Ag15889
• Nom complet: coiled-coil domain containing 50
• Masse moléculaire calculée: 482 aa, 56 kDa
• Poids moléculaire observé: 36-38 kDa
• Numéro d’acquisition GenBank: BC065004
• Symbole du gène: CCDC50
• Identification du gène (NCBI): 152137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326)

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