Anticorps Polyclonal de lapin anti-CCDC6

CCDC6 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 13717-1-AP

Synonymes

Papillary thyroid carcinoma-encoded protein, Coiled-coil domain-containing protein 6, coiled coil domain containing 6

---

Galerie de données de validation

• 

  WB analysis using 13717-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13717-1-AP (CCDC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 13717-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CCDC6 antibody (13717-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of MCF-7 using 13717-1-AP

  Immunofluorescent analysis of MCF-7 cells using 13717-1-AP (CCDC6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HeLa, cellules K-562, cellules MCF-7
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

13717-1-AP cible CCDC6 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCDC6 Protéine recombinante Ag4658
• Nom complet: coiled-coil domain containing 6
• Masse moléculaire calculée: 474 aa, 53 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC036757
• Symbole du gène: CCDC6
• Identification du gène (NCBI): 8030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Circular RNA circDNA2 upregulates CCDC6 expression to promote the progression of gastric cancer via miR-149-5p suppression.; Authors - Duochen Jin
human	WB	Mol Cancer Res; Dominant-Negative ATF5 Compromises Cancer Cell Survival by Targeting CEBPB and CEBPD.; Authors - Xiaotian Sun
human	WB	Genes (Basel); Insights into the Evolution of Spermatogenesis-Related Ubiquitin-Proteasome System Genes in Abdominal Testicular Laurasiatherians.; Authors - Xiaoyue Ding
rat	WB	Brain Res Bull; Attenuation of histone H4 lysine 16 acetylation (H4K16ac) elicits a neuroprotection against ischemic stroke by alleviating the autophagic/lysosomal dysfunction in neurons at the penumbra.; Authors - Dong Lingling
human	WB	Anal Bioanal Chem; A gene-encoded FRET fluorescent sensor designed for detecting asymmetric dimethylation levels in vitro and in living cells; Authors - Xuan Sun
human	WB	World J Gastroenterol; Suberoylanilide hydroxamic acid upregulates reticulophagy receptor expression and promotes cell death in hepatocellular carcinoma cells; Authors - Jia-Yao Li

{{ptg:RelatedPrimaryAntibodies}}