Anticorps Monoclonal anti-CCDC6

• Phare
• Validé par KD/KO

CCDC6 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D6A8

N° de cat : 67637-1-PBS

Synonymes

coiled coil domain containing 6, Coiled-coil domain-containing protein 6, Papillary thyroid carcinoma-encoded protein

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Galerie de données de validation

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  WB analysis using 67637-1-Ig (same clone as 67637-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67637-1-Ig (CCDC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67637-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67637-1-Ig (same clone as 67637-1-PBS)

  WB result of CCDC6 antibody (67637-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC6 transfected HepG2 cells. This data was developed using the same antibody clone with 67637-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67637-1-Ig (same clone as 67637-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67637-1-Ig (CCDC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67637-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67637-1-Ig (same clone as 67637-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67637-1-Ig (CCDC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67637-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67637-1-Ig (same clone as 67637-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CCDC6 antibody (67637-1-Ig, Clone: 1D6A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67637-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51465-1

  Cytometric bead array sample test of MP51465-1, CCDC6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67637-1-PBS. Detection antibody: 67637-2-PBS.

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  Cytometric bead array standard curve of MP51465-1

  Cytometric bead array standard curve of MP51465-1, CCDC6 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67637-1-PBS. Detection antibody: 67637-2-PBS. Standard:Ag6952. Range: 0.078-10 ng/mL.

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Informations sur le produit

67637-1-PBS cible CCDC6 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCDC6 Protéine recombinante Ag6952
• Nom complet: coiled-coil domain containing 6
• Masse moléculaire calculée: 474 aa, 53 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC036757
• Symbole du gène: CCDC6
• Identification du gène (NCBI): 8030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCDC6 (Coiled-coil domain-containing protein 6) is also named as Protein H4, D10S170 and TST1. CCDC6, was initially isolated as part of a tumorigenic DNA originated by the fusion of CCDC6 with the tyrosine kinase of RET receptor. CCDC6 has been considered as an accidental partner of the RET protooncogene, providing the promoter and the first 101 aa necessary for the constitutive activation of the oncogenic Tyrosine Kinase (TK) RET in thyroid cells. The 65 kDa product of CCDC6 has a nuclear transfer sequence with no transmembrane domains and is predicted to locate in both the nucleus and the cytoplasm (PMID: 29044514). The CCDC6 is a phosphoprotein, predicted target of several S/T kinases which can modulate the protein stability and the intracellular shuttling into the nucleus upon different cellular signals mediated by ERK1/2, ATM and CDK1/2. CCDC6 is involved in cellular response to DNA damage mediated by ATM, with the final result of promoting cellular apoptosis. CCDC6 depleted cells are considered defective of DNA repair checkpoint and proceed faster than the control cells in the cell cycle upon induced DNA damage (PMID: 22655027).

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