Anticorps Polyclonal de lapin anti-CCDC76

CCDC76 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31484-1-PBS

Synonymes

tRNA [Gm4] methyltransferase, TRMT13

---

Galerie de données de validation

• 

  WB analysis using 31484-1-AP (same clone as 31484-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31484-1-AP (CCDC76 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 31484-1-AP (same clone as 31484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 31484-1-AP (same clone as 31484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31484-1-AP (CCDC76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 31484-1-AP (same clone as 31484-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCDC76 antibody (31484-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 31484-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

31484-1-PBS cible CCDC76 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCDC76 Protéine recombinante Ag34110
• Nom complet: coiled-coil domain containing 76
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC075811
• Symbole du gène: CCDC76
• Identification du gène (NCBI): 54482
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCDC76 (coiled‐coil domain‐containing protein 76), also known as the human tRNA Nm4 methyltransferase (hTrmt13) is an uncharacterized human protein containing 481 amino acid residues that shares 27% sequence similarity with yeast Trm13p. The expression of CCDC76 was significantly associated with patient survival in breast carcinoma and liver and renal cancer patients (PMID: 34850409).

{{ptg:RelatedPrimaryAntibodies}}