Anticorps Polyclonal de lapin anti-CCDC85B

CCDC85B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18282-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse heart using 18282-1-AP (same clone as 18282-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18282-1-AP (CCDC85B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 18282-1-AP (same clone as 18282-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18282-1-AP (CCDC85B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 18282-1-AP (same clone as 18282-1-PBS)

  IP result of anti-CCDC85B (IP:18282-1-AP, 4ug; Detection:18282-1-AP 1:300) with mouse heart tissue lysate 3600ug. This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18282-1-AP (same clone as 18282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18282-1-AP (CCDC85B antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18282-1-AP (same clone as 18282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18282-1-AP (CCDC85B antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18282-1-AP (same clone as 18282-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18282-1-AP (CCDC85B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18282-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18282-1-PBS cible CCDC85B dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCDC85B Protéine recombinante Ag13129
• Nom complet: coiled-coil domain containing 85B
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 24-28 kDa
• Numéro d’acquisition GenBank: BC008796
• Symbole du gène: CCDC85B
• Identification du gène (NCBI): 11007
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Coiled-coil domain-containing protein 85B (CCDC85B), also named as DIPA, is 202 amino acid protein, which belongs to the CCDC85 family. CCDC85B localizes in the nucleus and cytoplasm. CCDC85B is widely expressed in many tissues and functions as a transcriptional repressor. CCDC85B may inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. CCDC85B may play a role in adipocyte differentiation.

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