Anticorps Recombinant de lapin anti-CCDC94

CCDC94 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240517A1

N° de cat : 83580-1-RR

Synonymes

YJU2, Coiled-coil domain-containing protein 94, 240517A1

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Galerie de données de validation

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  WB analysis using 83580-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83580-1-RR (CCDC94 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 83580-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-1-RR, Clone: 240517A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A549 using 83580-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-1-RR, Clone: 240517A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A431 using 83580-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug CCDC94 Recombinant antibody (83580-1-RR, Clone:240517A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83580-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83580-1-RR against Human CCDC94 were performed. The affinity constant is 1.08 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83580-1-RR cible CCDC94 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCDC94 Protéine recombinante Ag13764
• Nom complet: coiled-coil domain containing 94
• Masse moléculaire calculée: 323 aa, 37 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC000561
• Symbole du gène: CCDC94
• Identification du gène (NCBI): 55702
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

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