Anticorps Recombinant de lapin anti-CCDC94

CCDC94 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240517E9

N° de cat : 83580-5-PBS

Synonymes

YJU2, Coiled-coil domain-containing protein 94, 240517E9

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Galerie de données de validation

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  IHC staining of human intrahepatic cholangiocarcinoma using 83580-5-RR (same clone as 83580-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83580-5-RR (CCDC94 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83580-5-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 83580-5-RR (same clone as 83580-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CCDC94 antibody (83580-5-RR, Clone: 240517E9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83580-5-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83580-5-RR (same clone as 83580-5-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug CCDC94 Recombinant antibody (83580-5-RR, Clone:240517E9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83580-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00553-1

  Cytometric bead array standard curve of MP00553-1, CCDC94 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83580-5-PBS. Detection antibody: 83580-4-PBS. Standard: Ag13764. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83580-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83580-5-RR against Human CCDC94 were performed. The affinity constant is 1.02 nM.

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Informations sur le produit

83580-5-PBS cible CCDC94 dans les applications de IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CCDC94 Protéine recombinante Ag13764
• Nom complet: coiled-coil domain containing 94
• Masse moléculaire calculée: 323 aa, 37 kDa
• Numéro d’acquisition GenBank: BC000561
• Symbole du gène: CCDC94
• Identification du gène (NCBI): 55702
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CCDC94, also known as YJU2, is a splicing factor, predicted to be involved in RNA splicing and negative regulation of DNA damage response, signal transduction by p53 class mediator. Part of U2-type catalytic step 1 spliceosome. And that it is required for pre-mRNA splicing both in vivo and in vitro. Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome. (PMID: 17515604)

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