Anticorps Recombinant de lapin anti-CCKAR-specific

CCKAR-specific Recombinant Antibody for WB, IP, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242681A12

N° de cat : 85205-1-PBS

Synonymes

CCKAR, 242681A12, CCK A, CCK A receptor, CCK AR

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Galerie de données de validation

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  WB analysis using 85205-1-RR (same clone as 85205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85205-1-RR (CCKAR-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85205-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01958-1

  Sandwich ELISA standard curve of MP01958-1, Human CCKAR Recombinant Matched Antibody Pair - PBS only. 85205-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY00105. 85205-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP01958-1

  The mean CCKAR concentration was determined to be 4.0 ng/mL in HUVEC cell extract based on a 0.9 mg/mL extract load and 5.3 ng/mL in SGC-7901 cell extract based on a 2.4 mg/mL extract load.

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  IP experiment of mouse lung using 85205-1-RR (same clone as 85205-1-PBS)

  IP result of anti-CCKAR-specific (IP:85205-1-RR, 4ug; Detection:85205-1-RR 1:600) with mouse lung tissue lysate 1520 ug. This data was developed using the same antibody clone with 85205-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85205-1-PBS cible CCKAR-specific dans les applications de WB, IP, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cholecystokinin A receptor
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: NM_000730
• Symbole du gène: CCKAR
• Identification du gène (NCBI): 886
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cholecystokinin (CCK) receptor, including CCKAR and CCKBR, is a receptor for cholecystokinin, mainly regulating pancreatic growth and enzyme secretion, and smooth muscle contraction of the gallbladder and stomach (PMID: 34556863). CCKAR (Cholecystokinin receptor type A) is primarily expressed in the alimentary tract, while CCKBR is mainly found in the brain and the stomach (PMID: 16816139). CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer (PMID: 36733361). The calculated MW of CCKAR is 48 kDa, but the observed MW is around 75 kDa, which may be with glycosylation modification.

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